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ORIGINAL RESEARCH article
Front. Cardiovasc. Med.
Sec. Cardiovascular Pharmacology and Drug Discovery
Volume 12 - 2025 | doi: 10.3389/fcvm.2025.1512791
Integrating Network Pharmacology and Experimental Validation to Investigate the Mechanism of Gualou Xiebai Banxia Decoction Against Myocardial Ischemia
Provisionally accepted
Wanjun Zhu 1 Ping Shao 2
Ying Ren 1 Wenxuan Liu 1 Xiaorui Han 1 Kexin Zeng 1
Chunmei Dai 1* Feifei Liu 3*- 1 Jinzhou Medical University, Jinzhou, China
- 2 Benxi National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines Co.,Ltd., Benxi, China
- 3 First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning Province, China
Background: Gualou Xiebai Banxia Decoction (GXBD), a traditional Chinese medicine, is used to treat myocardial ischemia (MI). However, the molecular mechanisms underlying its effects remain unclear. This study integrated network pharmacology and experimental validation to investigate the efficacy and potential mechanisms of GXBD in MI treatment.Methods: Network pharmacology was used to predict the mechanism of action of GXBD in MI. The predicted results were verified using ECG, echocardiography, HE staining, TTC staining, DHE, JC-1, immunofluorescence, and Western blot analysis in an isoproterenol (ISO)-induced MI rat model.Network pharmacology identified 33 active components in GXBD and 139 potential targets against MI, with the PI3K/AKT signaling pathway playing a key role. Compared to the model group,
Keywords: Gualou Xiebai Banxia decoction, Mitochondrial dysfunction, Myocardial Ischemia, Network analysis, PI3K/AKT/Nrf2 signaling pathway
Received: 17 Oct 2024; Accepted: 27 Mar 2025.
Copyright: © 2025 Zhu, Shao, Ren, Liu, Han, Zeng, Dai and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Chunmei Dai, Jinzhou Medical University, Jinzhou, China
Feifei Liu, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning Province, China
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