AUTHOR=Yi Chenlong , Chen Fangyu , Ma Rongrong , Fu Zhi , Song Meijuan , Zhang Zhuan , Chen Lingdi , Tang Xing , Lu Peng , Li Ben , Zhang Qingfen , Song Qifeng , Zhu Guangzheng , Wang Wei , Wang Qiang , Wang Xiaowei 

TITLE=Serum level of calpains product as a novel biomarker of acute lung injury following cardiopulmonary bypass

JOURNAL=Frontiers in Cardiovascular Medicine

VOLUME=9

YEAR=2022

URL=https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2022.1000761

DOI=10.3389/fcvm.2022.1000761

ISSN=2297-055X

ABSTRACT=<sec><title>Objective</title><p>The aim of this study was to test the hypothesis whether serum level of calpains could become a meaningful biomarker for diagnosis of acute lung injury (ALI) in clinical after cardiac surgery using cardiopulmonary bypass (CPB) technology.</p></sec><sec><title>Methods and results</title><p>Seventy consecutive adults underwent cardiac surgery with CPB were included in this prospective study. Based on the American-European Consensus Criteria (AECC), these patients were divided into ALI (<italic>n</italic> = 20, 28.57%) and non-ALI (<italic>n</italic> = 50, 71.43%) groups. Serum level of calpains in terms of calpains’ activity which was expressed as relative fluorescence unit (RFU) per microliter and measured at beginning of CPB (baseline), 1 h during CPB, end of CPB as well as 1, 12, and 24 h after CPB. Difference of serum level of calpains between two groups first appeared at the end of CPB and remained different at subsequent test points. Univariate and multivariate logistic regression analysis indicated that serum level of calpains 1 h after CPB was an independent predictor for postoperative ALI (OR 1.011, 95% CI 1.001, 1.021, <italic>p</italic> = 0.033) and correlated with a lower PaO<sub>2</sub>/FiO<sub>2</sub> ratio in the first 2 days (The first day: r = -0.389, <italic>p</italic> &lt; 0.001 and the second day: r = -0.320, <italic>p</italic> = 0.007) as well as longer mechanical ventilation time (r = 0.440, <italic>p</italic> &lt; 0.001), intensive care unit (ICU) length of stay (LOS) (r = 0.419, <italic>p</italic> &lt; 0.001) and hospital LOS (r = 0.297, <italic>p</italic> = 0.013).</p></sec><sec><title>Conclusion</title><p>Elevated serum level of calpains correlate with impaired lung function and poor clinical outcomes, indicating serum level of calpains could act as a potential biomarker for postoperative ALI following CPB in adults.</p></sec><sec><title>Clinical trial registration</title><p>[<ext-link ext-link-type="uri" xlink:href="https://clinicaltrials.gov/show/NCT05610475" xmlns:xlink="http://www.w3.org/1999/xlink">https://clinicaltrials.gov/show/NCT05610475</ext-link>], identifier [NCT05610475].</p></sec>