This article is a correction to:
Adeno-Associated Virus-Mediated Gain-of-Function mPCSK9 Expression in the Mouse Induces Hypercholesterolemia, Monocytosis, Neutrophilia, and a Hypercoagulative State
Georgios Louloudis1,2
Samuele Ambrosini3
Francesco Paneni3,4,5
Giovanni G. Camici2,3
Dietmar Benke2,6
Jan Klohs1,2*- 1Institute for Biomedical Engineering, University of Zurich and ETH Zurich, Zurich, Switzerland
- 2Zurich Neuroscience Center (ZNZ), Zurich, Switzerland
- 3Center for Molecular Cardiology, University of Zurich, Zurich, Switzerland
- 4University Heart Center, Cardiology, University Hospital Zurich, Zurich, Switzerland
- 5Department of Research and Education, University Hospital Zurich, Zurich, Switzerland
- 6Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland
by Louloudis, G., Ambrosini, S., Paneni, F., Camici, G. G., Benke, D., and Klohs, J. (2021). Front Cardiovasc Med. 8:718741. doi: 10.3389/fcvm.2021.718741
In the original article, there was a mistake in Figure 1H as published. The graph incorrectly depicting higher serum HDL-cholesterol levels in control AAV mice on standard diet compared to the rest of the experimental groups. The corrected Figure 1H appears below. It depicts higher serum HDL-cholesterol levels in the mPCSK9-AAV mice on HFD compared to the rest of our experimental groups.
A correction has been made to Results, Elevated PCSK9 plasma levels and hypercholesterolemia induced by mPCSK9-AAV expression and intake of Western-type HFD, paragraph 2:
“Furthermore, mPCSK9-AAV injected mice on the HFD had elevated total, HDL-cholesterol and non-HDL cholesterol serum levels (Figures 1F–H).”
FIGURE 1
Figure 1. Verification of the mPCSK9-AAV mouse model of hypercholesterolemia. (A) Body weights of the four experimental groups: Control AAV + Standard diet (black circles, n = 12), Control AAV + HFD (black squares, n = 10), mPCSK9-AAV + Standard diet (gray upward triangles, n = 11), and mPCSK9-AAV + HFD (gray downward triangles, n = 10). Mean ± SD; repeated-measures ANOVA with Holm–Sidak post-hoc test;**p ≤ 0.01, ****p ≤ 0.0001. (B) Mouse PCSK9 plasma concentration in Control AAV + Standard diet (circles, n = 7), Control AAV + HFD (squares, n = 6), mPCSK9-AAV + Standard diet (upward triangles, n = 7), and mPCSK9-AAV + HFD (downward triangles, n = 7) groups. Data are the mean of three independent replicates. Mean ± SD; two-way ANOVA with Tukey's post-hoc test; ****p ≤ 0.0001. (C,D) Hepatic Ldl receptor (Ldlr) levels in control-injected (n = 6) and mPCSK9-AAV-injected mice (n = 6) on standard diet normalized to total protein. Data are the mean of three independent replicates. Two gels were loaded per replicate. Mean ± SD; unpaired t-test; ****p ≤ 0.0001. (E) Representative images of Oil-red O-stained thoracic aortae of mPCSK9-AAV and control AAV mice on HFD. Mice did not develop atherosclerotic lesions in the selected time frame. Scale bar, 2 mm. (F–H) Serum total cholesterol (F), non-HDL cholesterol (G), and HDL cholesterol (H) in the different groups: Control AAV + Standard diet (n = 6), Control AAV + HFD (n ≥ 8), mPCSK9-AAV + Standard diet (n ≥ 9), and mPCSK9-AAV + HFD (n ≥ 7). Mean ± SD; two-way ANOVA with Tukey's post-hoc test; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. AAV, adeno-associated virus; HFD, high-fat diet; HDL, high-density lipoprotein.
Furthermore, a correction has been made to the Discussion, paragraph 3:
“Concomitantly, the non-HDL-cholesterol and HDL-cholesterol concentrations were significantly higher in mPCSK9-AAV mice on HFD compared to control AAV mice on standard diet (Figures 1G,H).”
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: PCSK9, hypercholesterolemia, mouse, neutrophils, monocytes, coagulation
Citation: Louloudis G, Ambrosini S, Paneni F, Camici GG, Benke D and Klohs J (2022) Corrigendum: Adeno-Associated Virus-Mediated Gain-of-Function mPCSK9 Expression in the Mouse Induces Hypercholesterolemia, Monocytosis, Neutrophilia, and a Hypercoagulative State. Front. Cardiovasc. Med. 8:820282. doi: 10.3389/fcvm.2021.820282
Received: 22 November 2021; Accepted: 20 December 2021;
Published: 12 January 2022.
Edited and reviewed by: Gabrielle Fredman, Albany Medical College, United States
Copyright © 2022 Louloudis, Ambrosini, Paneni, Camici, Benke and Klohs. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Jan Klohs, klohs@biomed.ee.ethz.ch; orcid.org/0000-0003-4065-2807