AUTHOR=Le D. Elizabeth , García-Jaramillo Manuel , Bobe Gerd , Alcazar Magana Armando , Vaswani Ashish , Minnier Jessica , Jump Donald B. , Rinkevich Diana , Alkayed Nabil J. , Maier Claudia S. , Kaul Sanjiv 

TITLE=Plasma Oxylipins: A Potential Risk Assessment Tool in Atherosclerotic Coronary Artery Disease

JOURNAL=Frontiers in Cardiovascular Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2021.645786

DOI=10.3389/fcvm.2021.645786

ISSN=2297-055X

ABSTRACT=<p><bold>Background:</bold> While oxylipins have been linked to coronary artery disease (CAD), little is known about their diagnostic and prognostic potential.</p><p><bold>Objective:</bold> We tested whether plasma concentration of specific oxylipins may discriminate among number of diseased coronary arteries and predict median 5-year outcomes in symptomatic adults.</p><p><bold>Methods:</bold> Using a combination of high-performance liquid chromatography (HPLC) and quantitative tandem mass spectrometry, we conducted a targeted analysis of 39 oxylipins in plasma samples of 23 asymptomatic adults with low CAD risk and 74 symptomatic adults (≥70% stenosis), aged 38–87 from the Greater Portland, Oregon area. Concentrations of 22 oxylipins were above the lower limit of quantification in &gt;98% of adults and were compared, individually and in groups based on precursors and biosynthetic pathways, in symptomatic adults to number of diseased coronary arteries [(1) <italic>n</italic> = 31; (2) <italic>n</italic> = 23; (3) <italic>n</italic> = 20], and outcomes during a median 5-year follow-up (no surgery: <italic>n</italic> = 7; coronary stent placement: <italic>n</italic> = 24; coronary artery bypass graft surgery: <italic>n</italic> = 26; death: <italic>n</italic> = 7).</p><p><bold>Results:</bold> Plasma levels of six quantified oxylipins decreased with the number of diseased arteries; a panel of five oxylipins diagnosed three diseased arteries with 100% sensitivity and 70% specificity. Concentrations of five oxylipins were lower and one oxylipin was higher with survival; a panel of two oxylipins predicted survival during follow-up with 86% sensitivity and 91% specificity.</p><p><bold>Conclusions:</bold> Quantification of plasma oxylipins may assist in CAD diagnosis and prognosis in combination with standard risk assessment tools.</p>