AUTHOR=Chen Ningyuan , Miao Liu , Lin Wei , Zou Donghua , Huang Ling , Huang Jia , Shi Wanxin , Li Lilin , Luo Yuxing , Liang Hao , Pan Shangling , Peng Junhua 

TITLE=Integrated DNA Methylation and Gene Expression Analysis Identified S100A8 and S100A9 in the Pathogenesis of Obesity

JOURNAL=Frontiers in Cardiovascular Medicine

VOLUME=8

YEAR=2021

URL=https://www.frontiersin.org/journals/cardiovascular-medicine/articles/10.3389/fcvm.2021.631650

DOI=10.3389/fcvm.2021.631650

ISSN=2297-055X

ABSTRACT=<p><bold>Background:</bold> To explore the association of DNA methylation and gene expression in the pathology of obesity.</p><p><bold>Methods:</bold> (1) Genomic DNA methylation and mRNA expression profile of visceral adipose tissue (VAT) were performed in a comprehensive database of gene expression in obese and normal subjects. (2) Functional enrichment analysis and construction of differential methylation gene regulatory networks were performed. (3) Validation of the two different methylation sites and corresponding gene expression was done in a separate microarray dataset. (4) Correlation analysis was performed on DNA methylation and mRNA expression data.</p><p><bold>Results:</bold> A total of 77 differentially expressed mRNAs matched with differentially methylated genes. Analysis revealed two different methylation sites corresponding to two unique genes—s100a8-cg09174555 and s100a9-cg03165378. Through the verification test of two interesting different expression positions [differentially methylated positions (DMPs)] and their corresponding gene expression, we found that methylation in these genes was negatively correlated to gene expression in the obesity group. Higher S100A8 and S100A9 expressions in obese subjects were validated in a separate microarray dataset.</p><p><bold>Conclusion:</bold> This study confirmed the relationship between DNA methylation and gene expression and emphasized the important role of S100A8 and S100A9 in the pathogenesis of obesity.</p>