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ORIGINAL RESEARCH article

Front. Bioinform.
Sec. RNA Bioinformatics
Volume 4 - 2024 | doi: 10.3389/fbinf.2024.1493712

In Silico Identification of chilli Genome encoded MicroRNAs Targeting the 16S rRNA and secA genes of 'Candidatus Phytoplasma trifolii'

Provisionally accepted
VINEETA PANDEY VINEETA PANDEY 1Aarshi Srivastava Aarshi Srivastava 1Ramwant Gupta Ramwant Gupta 1Muhammad Shafiq Shahid Muhammad Shafiq Shahid 2RAJARSHI KUMAR Gaur RAJARSHI KUMAR Gaur 1*
  • 1 Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India
  • 2 Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Muscat, Oman

The final, formatted version of the article will be published soon.

    Phytoplasma, a potentially hazardous pathogen associated with witches' broom, is an economically harmful disease-producing bacteria that damages chilli cultivation. Phytoplasma-infected plants display various symptoms that indicate significant disruptions in normal plant physiology and behaviour.Diseases caused by phytoplasma are widespread and have a major economic impact on crop quality and yield. This work focuses on identifying and examining chilli microRNAs (miRNAs) as potential targets against the 16S rRNA and secA gene of 'Candidatus Phytoplasma trifolii' ('Ca. P. trifolii') through plant miRNA prediction algorithms. Mature chilli miRNAs (CA-miRNAs) were collected and used to hybridise the 16S rRNA and secA genes. A total of four common CA-miRNAs were picked according to genetic consensus. Three algorithms applied in the present study suggested that the physiologically relevant, top-ranked miR169b_2 has a possibly specific site at nucleotide position 1006 for targeting the 'Ca. P. trifolii' 16S rRNA gene. The circos algorithm was then utilised to create the miRNA-mRNA regulatory network. The free energy between the miRNA:mRNA duplex was also computed, and the best value of -17.46 Kcal/mol was obtained for CA-miR166c_2. Currently, there are no suitable commercial 'Ca. P. trifolii'-resistant chilli crops. As a result, the expected biological data provide useful evidence for developing 'Ca. P. trifolii'-resistant chilli plants.

    Keywords: Phytoplasma, 'Candidatus Phytoplasma trifolii', Chilli, 16S rRNA, SecA, miRNA

    Received: 09 Sep 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 PANDEY, Srivastava, Gupta, Shafiq Shahid and Gaur. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: RAJARSHI KUMAR Gaur, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, India

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.