Harrison W Cayatineto Shazia T Hakim ^*

• Diné College, Tsaile, United States

Introduction: Flaviviridae are a group of enveloped, positive stranded RNA viruses mainly transmitted through either mosquitoes or tick bites and/or contaminated blood, blood products or other body secreations. These viruses cause diseases ranging from mild to severe and are considered important human pathogens. MicroRNAs (miRNA) are non-coding molecules involved in growth, development, cell proliferations, protein synthesis, apoptosis, and pathogenesis. These small molecules are even being used as gene suppressers in antiviral therapeutics, inhibiting viral replication. In current study, we used bioinformatic tools to predict a possible miRNA sequence that could be complementary to the nucleocapsid (NP) and/or capsid (CP) gene of the Flaviviridae family and provide an inhibitory solution. Methods:Bioinformatics is a field of science that include tremendous computational analysis, logarithms, and sequence alignments. To predict the right alignments between miRNA and viral mRNA genomes, we used computational databases such as miRBase, NCBI, and Basic Alignment Search Tools -nucleotides (BLAST-n). Results: Out of 2,600 mature miRNAs, hsa-miR-548d-3p revealed complementary seuqences with the flaviviruses capsid gene and BVDV virus capsid gene and was selected as a possible candidate to inhibit flaviviruses. Conclusion: Although more detailed in-vitro and in-vivo studies are required to test the possible inhibitiory effects of hsa-miR-548d-3p against flaviviruses, this computational study may be the first step to study further developing a novel therapeutic for lethal viruses within the Flavivirdae family using suggested candidate miRNA.