AUTHOR=Davis M. Wayne , Jorgensen Erik M. 

TITLE=ApE, A Plasmid Editor: A Freely Available DNA Manipulation and Visualization Program

JOURNAL=Frontiers in Bioinformatics

VOLUME=2

YEAR=2022

URL=https://www.frontiersin.org/journals/bioinformatics/articles/10.3389/fbinf.2022.818619

DOI=10.3389/fbinf.2022.818619

ISSN=2673-7647

ABSTRACT=<p>A plasmid Editor (ApE) is a free, multi-platform application for visualizing, designing, and presenting biologically relevant DNA sequences. ApE provides a flexible framework for annotating a sequence manually or using a user-defined library of features. ApE can be used in designing plasmids and other constructs <italic>via in silico</italic> simulation of cloning methods such as PCR, Gibson assembly, restriction-ligation assembly and Golden Gate assembly. In addition, ApE provides a platform for creating visually appealing linear and circular plasmid maps. It is available for Mac, PC, and Linux-based platforms and can be downloaded at <ext-link ext-link-type="uri" xlink:href="https://jorgensen.biology.utah.edu/wayned/ape/" xmlns:xlink="http://www.w3.org/1999/xlink">https://jorgensen.biology.utah.edu/wayned/ape/</ext-link>.</p>