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ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Nanobiotechnology
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1570668
This article is part of the Research TopicThe Road toward Nano-Based Diagnostics for Health and DiseaseView all 9 articles
A ultra-stable point of care nanozyme-based kit for cTnI detection in human serum
Provisionally accepted- 1The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- 2The First Affiliated Hospital of Henan University, Kaifeng, China
- 3Joint National Laboratory for Antibody Drug Engineering, Henan University, Kaifeng, China
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Home discovery of myocardial infarction can significantly improve the rate of treatment. Cardiac troponin I (cTnI), as a biochemical marker that has been introduced into clinical diagnosis and treatment guidelines, can effectively predict the occurrence of myocardial infarction. We constructed highly stable nanozyme based on Fe3O4 nanoparticles and prepared rapid detection reagents for myocardial infarction by modifying anti-cTnI antibodies. The results showed that the nanozyme with an average particle size of 200 nm had peroxidase activity and could effectively catalyze 3,3 ', 5,5 'tetramethylbenzidine (TMB). With a sensitivity of up to 1.5 ng/mL, the kit showed superior performance than commercial colloidal gold assay kits, and could effectively detect cTnI in serum with an overall compliance rate of 92.1%. The study provides a new approach to home detection of heart attacks.
Keywords: cTnI, Iron oxide superparamagnetic nanoparticles, Nanozyme, Lateral flow immunoassay, acute myocardial infarction
Received: 04 Feb 2025; Accepted: 07 Apr 2025.
Copyright: © 2025 Sun, Yang, Wu, Ma, Tao and Lu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Shaoying Lu, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
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