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ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Biomaterials
Volume 13 - 2025 |
doi: 10.3389/fbioe.2025.1551791
This article is part of the Research Topic Functional Biomaterials and Seed Cells in Tissue Engineering View all articles
Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs
Provisionally accepted
Jiangru An 1* Jinyi Zhang 1* Qiuhua Wang 1* Tianyi Ma 2*
Paul Santerre 3 Wenshuang Wang 4* Peng Ma 1*
Xiaoqing Zhang 1*- 1 International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, China
- 2 The University of Hong Kong, Pokfulam, Hong Kong, SAR China
- 3 University of Toronto, Toronto, Ontario, Canada
- 4 Yantai Yuhuangding Hospital, Yantai, Shandong Province, China
Human endometrial mesenchymal stem cells (H-EMSCs) can inhibit endometrial fibrosis and repair damaged endometrium. However, direct cell injection into damaged endometrium shows limited cell survival. Cell seeding onto biomaterial-based electrospun membranes could improve H-EMSCs' survival and prolong their stay at the damaged endometrium. Polycaprolactone (PCL), silk fibroin (SF) and hyaluronic acid (HA) are synthetic or natural biomaterials used by the biomedicine field, however, their effects on the biological activities of H-EMSCs remain unclear. In this study, CD90 + CD73 + CD45 -H-EMSCs were extracted from human endometrium and H-EM-SCs showed enhanced adhesion, proliferation on PCL-HA vs. PCL, PCL-SF, establishing the potential of the composite to address cell survival issues. H-EMSCs cultured on PCL-HA showed decreased IL-6 gene expression and increased IL-10, VEGFA, TGFβ gene expression vs. PCL-SF, establishing the potential to create a favorable microenvironment for generating vascularized endometrial tissues. PCL, PCL-SF, PCL-HA all supported CD90 and Meflin expression of the seeded H-EMSCs, establishing PCL as a platform to form enhanced biomaterial composites for endometrial repair in the future. This study provided significant evidence supporting the potential of appropriately tailored composites of PCL and HA to moderate inflammation and wound-healing, which can be applied for endometrial tissue repair and regeneration.
Keywords: human endometrial mesenchymal stem cells, biomaterial electrospun membranes, Poly-caprolactone, silk fibroin, Hyaluronic Acid
Received: 26 Dec 2024; Accepted: 03 Feb 2025.
Copyright: © 2025 An, Zhang, Wang, Ma, Santerre, Wang, Ma and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jiangru An, International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, China
Jinyi Zhang, International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, China
Qiuhua Wang, International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, China
Tianyi Ma, The University of Hong Kong, Pokfulam, 999077, Hong Kong, SAR China
Wenshuang Wang, Yantai Yuhuangding Hospital, Yantai, Shandong Province, China
Peng Ma, International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, China
Xiaoqing Zhang, International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, China
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