BMP2 peptide-modified polycaprolactone-collagen nanosheets for periodontal tissue regeneration

Yong Zhang ¹ Junxian Ren ¹ Zongshan Shen ¹ Jiayu Yang ¹ Jichen Yang ¹ Zhengmei Lin ¹ Xuetao Shi ² Chuanjiang Zhao ¹ Juan Xia ^1*

• ¹ Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong Province, China
• ² Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou, Guangdong Province, China

Periodontitis degrades tooth-supporting tissues, leading to tooth mobility and loss.Guided tissue regeneration (GTR) surgery uses barrier membranes to promote tissue regeneration, but conventional membranes lack bone-inducing properties, limiting their effectiveness. To address this, we developed BMP2 peptide-modified polycaprolactone-collagen nanosheets (BPCNs) for GTR. BPCNs consist of a bioactive collagen layer modified with BMP2 peptide to enhance cell adhesion and bone regeneration, supported by a hydrophobic PCL layer for barrier function and mechanical strength. BPCNs exhibit excellent biocompatibility, promote fibroblast and bone marrow stem cell (BMSC) adhesion, and enhance BMSC osteogenesis. Furthermore, BPCNs significantly promoted periodontal tissue regeneration in a rat model. Mechanistically, RNA sequencing revealed that BPCNs upregulated genes involved in tissue regeneration and downregulated proinflammatory pathways. These findings suggest BPCNs as a promising material for GTR, offering bone-inducing properties and enhancing periodontal tissue regeneration.