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ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Biosensors and Biomolecular Electronics
Volume 13 - 2025 | doi: 10.3389/fbioe.2025.1499164
This article is part of the Research Topic Sensing Technologies for Sustainable Healthcare View all 6 articles
Detection of Testosterone Based on the Interaction Between Variable Regions of Antibody Heavy Chain and Light Chain
Provisionally accepted- 1 School of Basic Medical Sciences, Shandong University, Jinan, Shandong Province, China
- 2 University of Health and Rehabilitation Sciences, Qingdao, China
- 3 Shandong Second Medical University, Weifang, Shandong Province, China
- 4 International Research Frontiers Initiative, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan
Testosterone is a steroid hormone, which plays a pivotal role in regulating metabolism and protein synthesis in the body. The detection of testosterone is of paramount importance for diagnostic purposes in clinical settings, as well as for monitoring athlete performance and ensuring the integrity of sports competitions. Here, a sensitive non-competitive open sandwich enzyme-linked immunosorbent assay (OS-ELISA) was developed, featuring high sensitivity, broad detection range, and straightforward operation, for the quantification of testosterone. The OS-ELISA is based on the principle of enhanced interaction between antigen-driven antibody variable regions. An enhanced OS-ELISA utilizing purified maltose-binding proteinfused VL (MBP-VL) and VH phages demonstrated a LOD of 1.07 pM and a wide working range from 1 pM to 10 mM.
Keywords: No.2 OS-ELISA, Testosterone, phage display, Rapid detection, antibody
Received: 20 Sep 2024; Accepted: 04 Mar 2025.
Copyright: © 2025 Zhao, Zhang, Zou, Jia, Zheng and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jinhua Dong, University of Health and Rehabilitation Sciences, Qingdao, China
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