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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Bioprocess Engineering
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1521223
This article is part of the Research Topic Isolation, Modification, and Characterization of the Constituents in Biomass and Their Bio-based Applications - Volume III View all 6 articles

Novel one-step lignin microsphere preparation for oral tissue regeneration application

Provisionally accepted
Anyuan Shi Anyuan Shi 1Li Guo Li Guo 1Chunning Gu Chunning Gu 1Yunni Zhan Yunni Zhan 2Xuelian Zhou Xuelian Zhou 2*Wei Cheng Wei Cheng 1*
  • 1 Nanjing Stomatological Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu Province, China
  • 2 Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, China

The final, formatted version of the article will be published soon.

    Lignin is a naturally derived biomacromolecule, which shows excellent biocompatibility and has the potential for the biomedical application. For the first time, this study isolated a nanosized lignin microspheres (LMS) directly from wheat straw by a polyol-based deep eutectic solvent. The size of these LMS can be regulated by changing the isolation parameters, ranging from 90 to 330 nm. Structures of these LMSs was comprehensively investigated by SEM, GPC, HSQC NMR and 31 P NMR, which explained the formation mechanism of the hydrophobicity-induced selfassembly. The LMS shows good antioxidation of 52.99%-76.26% towards DPPH. In vitro biomedical tests further revealed that the LMS at concentration <25 μg/mL had good biocompatibility towards gingival mesenchymal stem cells (GMSCs) and jaw bone marrow mesenchymai stem cells (JBMMSCs), with low apoptosis rate, outperforming other lignin materials. The presented results highlighted the application of the nanosized LMS as a potential biomaterial in the oral tissue regeneration.

    Keywords: Deep eutectic solvent, lignin microspheres, antioxidant activity, in vitro biomedical test, Biocompatibility

    Received: 01 Nov 2024; Accepted: 13 Dec 2024.

    Copyright: © 2024 Shi, Guo, Gu, Zhan, Zhou and Cheng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xuelian Zhou, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, China
    Wei Cheng, Nanjing Stomatological Hospital, School of Medicine, Nanjing University, Nanjing, 210008, Jiangsu Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.