The final, formatted version of the article will be published soon.
ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Industrial Biotechnology
Volume 12 - 2024 |
doi: 10.3389/fbioe.2024.1493766
Analysis of the Effects of Bacillus velezensis HJ-16 Inoculation on Tobacco Leaves Based on Multi-omics Methods
Provisionally accepted
Zhou Qing
1,2
Yang Jin chu
1*
Feng Ying jie
1*
Yang Zong can
1*
Wang Yi xuan
1*
Zhang Zhan
1*
Zhang Ting ting
1
Liu Wen zhao
1*
Xu Yong ming
1*
Yang Yong feng
1*
Huang Ji hong
2*- 1 China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan Province, China
- 2 Henan University, Kaifeng, China
In this study, a strain isolated from the surface of flue-cured tobacco leaves, identified as Bacillus velezensis HJ-16, was applied in the solid-state fermentation of tobacco leaves. This strain, known for producing thermally stable enzymes, including amylase, cellulase, and protease, significantly improved the sensory qualities of tobacco, enhancing aromatic intensity, density, and softness, while reducing irritation. Whole-genome sequencing and functional annotation revealed that B. velezensis HJ-16 possesses a single circular chromosome containing genes associated with enzyme production and metabolic activities, particularly in carbohydrate metabolism and amino acid metabolism. Untargeted metabolomics analysis identified significant changes in non-volatile metabolites induced by fermentation. These metabolites were enriched in pathways related to flavonoid biosynthesis, alkaloid biosynthesis, aromatic amino acid metabolism, lipid metabolism, and carbon metabolism. Metagenomic analysis showed that Bacillus became the dominant genus on the tobacco leaf surface following inoculation with B. velezensis HJ-16, altering the microbial community composition, reducing diversity and evenness, and enhancing microbial metabolic activity. These findings underscore the potential of B. velezensis HJ-16 as a biotechnological tool to improve tobacco leaf quality.
Keywords: Tobacco, Bacillus velezensis, Fermentation, Whole-genome sequencing, Metagenomic, untargeted metabolomics
Received: 09 Sep 2024; Accepted: 18 Nov 2024.
Copyright: © 2024 Qing, chu, jie, can, xuan, Zhan, ting, zhao, ming, feng and hong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Yang Jin chu, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Feng Ying jie, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Yang Zong can, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Wang Yi xuan, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Zhang Zhan, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Liu Wen zhao, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Xu Yong ming, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Yang Yong feng, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
Huang Ji hong, Henan University, Kaifeng, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.