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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Industrial Biotechnology
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1493766

Analysis of the Effects of Bacillus velezensis HJ-16 Inoculation on Tobacco Leaves Based on Multi-omics Methods

Provisionally accepted
Zhou Qing Zhou Qing 1,2Yang Jin chu Yang Jin chu 1*Feng Ying jie Feng Ying jie 1Yang Zong can Yang Zong can 1*Wang Yi xuan Wang Yi xuan 1*Zhang Zhan Zhang Zhan 1*Zhang Ting ting Zhang Ting ting 1Liu Wen zhao Liu Wen zhao 1Xu Yong ming Xu Yong ming 1*Yang Yong feng Yang Yong feng 1*Huang Ji hong Huang Ji hong 2*
  • 1 China Tobacco Henan Industrial Co., Ltd., Zhengzhou, Henan Province, China
  • 2 Henan University, Kaifeng, China

The final, formatted version of the article will be published soon.

    In this study, a strain isolated from the surface of flue-cured tobacco leaves, identified as Bacillus velezensis HJ-16, was applied in the solid-state fermentation of tobacco leaves. This strain, known for producing thermally stable enzymes, including amylase, cellulase, and protease, significantly improved the sensory qualities of tobacco, enhancing aromatic intensity, density, and softness, while reducing irritation. Whole-genome sequencing and functional annotation revealed that B. velezensis HJ-16 possesses a single circular chromosome containing genes associated with enzyme production and metabolic activities, particularly in carbohydrate metabolism and amino acid metabolism. Untargeted metabolomics analysis identified significant changes in non-volatile metabolites induced by fermentation. These metabolites were enriched in pathways related to flavonoid biosynthesis, alkaloid biosynthesis, aromatic amino acid metabolism, lipid metabolism, and carbon metabolism. Metagenomic analysis showed that Bacillus became the dominant genus on the tobacco leaf surface following inoculation with B. velezensis HJ-16, altering the microbial community composition, reducing diversity and evenness, and enhancing microbial metabolic activity. These findings underscore the potential of B. velezensis HJ-16 as a biotechnological tool to improve tobacco leaf quality.

    Keywords: Tobacco, Bacillus velezensis, Fermentation, Whole-genome sequencing, Metagenomic, untargeted metabolomics

    Received: 09 Sep 2024; Accepted: 18 Nov 2024.

    Copyright: © 2024 Qing, chu, jie, can, xuan, Zhan, ting, zhao, ming, feng and hong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Yang Jin chu, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Yang Zong can, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Wang Yi xuan, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Zhang Zhan, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Xu Yong ming, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Yang Yong feng, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, 450016, Henan Province, China
    Huang Ji hong, Henan University, Kaifeng, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.