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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Biomaterials
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1468296
This article is part of the Research Topic Biomaterials and Biological Regulation for Bone Tissue Remodeling and Regeneration View all 5 articles

Effect of α-calcium sulphate hemihydrate /treated detin matrix composite to regenerate bone in critically sized SD-Rat calvarial defects

Provisionally accepted
Mengmeng Wang Mengmeng Wang 1Haojie Fu Haojie Fu 2Bingyan Li Bingyan Li 2Wenshuai Li Wenshuai Li 2Zelong Hu Zelong Hu 2Rui Li Rui Li 2*
  • 1 Jiading District Central Hospital Affiliated Shanghai University of Medicine &Health Sciences, shanghai, China
  • 2 First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

The final, formatted version of the article will be published soon.

    Although α-calcium sulphate hemihydrate(α-CSH) has been widely used as an artificial bone graft material, it has several drawbacks, such as quick deterioration and limited osteoinductivity. Extensive research is now being conducted to produce advanced biomaterials based on α-CSH that has enhanced osteogenic properties for the healing of bone deformities. The aim of this work was to create a mixture of treated dentin matrix (TDM) and α-CSH to produce composite cements, and then examine their potential to promote the development of new bone in rats with critical-sized calvaria bone lesions. The characteristics of TDM /α-CSH composites were assessed using a universal mechanical testing machine, scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The biological characteristics of TDM /α-CSH composites were investigated by measuring the development and proliferation of osteoblasts using the CCK-8 test. In addition, the cements were implemented in the restoration of calvaria bone defects in rodents. The results of our study indicated that the compressive strength of the TDM/α-CSH cements exhibited a decline as the TDM mass ratio increased. Upon thorough mixing, the scanning electron microscopy analysis revealed that TDM/α-CSH had a distinct three-dimensional porous network structure and a notable enhancement in surface roughness. The XRD and FT-IR studies confirmed that the combination of the two materials resulted in a physical mixture, and the composition of the phases in the composites remained unchanged. The TDM/α-CSH composites demonstrated a significant stimulatory effect on osteoblast proliferation, which was dependent on the amount of TDM incorporated. Furthermore, in an in vivo experiment using a calvarial bone defect model, TDM/α-CSH cements exhibited superior enhancement of new bone formation, as confirmed by X-ray examination and μ-CT analysis. These findings suggest that TDM/α-CSH composite cements have promising potential as an alternative for repairing bone defects.

    Keywords: treated dentin matrix, Bone, stem cell, Tissue Engineering, Bone regenaration

    Received: 21 Jul 2024; Accepted: 18 Nov 2024.

    Copyright: © 2024 Wang, Fu, Li, Li, Hu and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Rui Li, First Affiliated Hospital of Zhengzhou University, Zhengzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.