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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Bioprocess Engineering
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1461343
This article is part of the Research Topic Isolation, Modification, and Characterization of the Constituents in Biomass and Their Bio-based Applications - Volume III View all articles

Effect of H2O2-VC degradation on structural characteristics and immunomodulatory activity of larch arabinogalactan

Provisionally accepted
Qiang Yong Qiang Yong 1*Huimin Qi Huimin Qi 1Shuo Tang Shuo Tang 2Bin Bian Bin Bian 1Chenhuan Lai Chenhuan Lai 1Yanan Chen Yanan Chen 1Zhe Ling Zhe Ling 1
  • 1 Nanjing Forestry University, Nanjing, China
  • 2 Nanjing Institute for Comprehensive Utilization of Wild Plants, China CO-OP, Nanjing, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    The arabinogalactan in the representative softwood biomass of larch was degraded using an environmentally friendly hydrogen peroxide and vitamin C (H2O2-VC) system to improve its immunomodulatory activity. Through the H2O2-VC degradation mechanism, hydroxyl radicals are generated, which then target the hydrogen atoms within polysaccharides, resulting in the breaking of glycosidic bonds. Given the impact of oxidative degradation on polysaccharides, we identified three specific arabinogalactan degradation products distinguished by their arabinosyl side chain compositions. The primary structures of the degradation products were investigated using Fourier-transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. Congo red staining showed that the degradation products were absent in the triple-helix structure. The results of the in vitro immunological experiments indicated that an appropriate reduction in the molar ratio of arabinose to galactose enhanced the immunostimulatory effects on RAW 264.7 cells. In addition, the immunostimulatory pathway mediated by arabinogalactan was explored by toll-like receptor 4 (TLR4) inhibitor (TAK-242) These findings provide novel insights into the understanding of the relationship between the structure of arabinogalactan and its biological activity.

    Keywords: Arabinogalactan, degradation, H2O2-VC, Structural characteristics, Immunomodulatory activity

    Received: 08 Jul 2024; Accepted: 29 Jul 2024.

    Copyright: © 2024 Yong, Qi, Tang, Bian, Lai, Chen and Ling. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Qiang Yong, Nanjing Forestry University, Nanjing, China

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