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ORIGINAL RESEARCH article
Front. Bioeng. Biotechnol.
Sec. Tissue Engineering and Regenerative Medicine
Volume 12 - 2024 |
doi: 10.3389/fbioe.2024.1460623
This article is part of the Research Topic A New Age for Articular Cartilage: from Bench to Beside of Tissue Engineering and Regenerative Medicine in Cartilage Tissue Repair View all 5 articles
Application of Polydopamine-Modified Triphasic PLA/PCL-PLGA/Mg(OH)2-Velvet antler polypeptides Scaffold Loaded with Fibrocartilage Stem Cells for the Repair of Osteochondral Defects
Provisionally accepted- 1 Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
- 2 Yunnan Key Laboratory of Stomatology, Kunming, China
- 3 Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
- 4 Second Clinic, Kunming Medical University Affiliated Stomatological Hospital, Kunming, China
Articular cartilage defects often involve damage to both the cartilage and subchondral bone, requiring a scaffold that can meet the unique needs of each tissue type and establish an effective barrier between the bone and cartilage. In this study, we used 3D printing technology to fabricate a tri-phasic scaffold composed of PLA/PCL-PLGA/Mg(OH)₂, which includes a cartilage layer, an osteochondral interface, and a bone layer. The scaffold was filled with Velvet antler polypeptides (VAP), and its characterization was assessed using compression testing, XRD, FTIR, SEM, fluorescence microscopy, and EDS. In vitro investigation demonstrated that the scaffold not only supported osteogenesis but also promoted chondrogenic differentiation of fibrocartilage stem cells (FCSCs). n vivo experiments showed that the tri-phasic PLA/PCL-PLGA/Mg(OH)2-VAP scaffold together with FCSC, when transplanted to animal models, increased the recovery of osteochondral defects. Those results demonstrate the promising future of illustrated tri-phasic PLA/PCL-PLGA/Mg(OH)2-VAP scaffold loaded with FCSCs as a new bone and cartilage tissue engineering approach for osteochondral defects treatment.
Keywords: Fibrocartilage stem cells, Velvet antler polypeptide, Tissue Engineering, Triphasic scaffold, osteochondral defects
Received: 06 Jul 2024; Accepted: 06 Sep 2024.
Copyright: © 2024 Renyi, Tao, Ma, Peishen, Chaofeng, Yuchen, Changyu, jinjun and Yanhua. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Deng Peishen, Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
Liu Chaofeng, Second Clinic, Kunming Medical University Affiliated Stomatological Hospital, Kunming, China
Hong Yuchen, Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
Liu Changyu, Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
Tian jinjun, Yunnan Key Laboratory of Stomatology, Kunming, China
Xu Yanhua, Yunnan Key Laboratory of Stomatology, Kunming, China
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