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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Tissue Engineering and Regenerative Medicine
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1460623
This article is part of the Research Topic A New Age for Articular Cartilage: from Bench to Beside of Tissue Engineering and Regenerative Medicine in Cartilage Tissue Repair View all 5 articles

Application of Polydopamine-Modified Triphasic PLA/PCL-PLGA/Mg(OH)2-Velvet antler polypeptides Scaffold Loaded with Fibrocartilage Stem Cells for the Repair of Osteochondral Defects

Provisionally accepted
Cheng Renyi Cheng Renyi 1,2Xie Tao Xie Tao 1,2Wen Ma Wen Ma 2,3Deng Peishen Deng Peishen 1,2*Liu Chaofeng Liu Chaofeng 2,4*Hong Yuchen Hong Yuchen 1,2*Liu Changyu Liu Changyu 1,2*Tian jinjun Tian jinjun 1,2*Xu Yanhua Xu Yanhua 1,2*
  • 1 Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
  • 2 Yunnan Key Laboratory of Stomatology, Kunming, China
  • 3 Department of Oral and Maxillofacial Surgery, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
  • 4 Second Clinic, Kunming Medical University Affiliated Stomatological Hospital, Kunming, China

The final, formatted version of the article will be published soon.

    Articular cartilage defects often involve damage to both the cartilage and subchondral bone, requiring a scaffold that can meet the unique needs of each tissue type and establish an effective barrier between the bone and cartilage. In this study, we used 3D printing technology to fabricate a tri-phasic scaffold composed of PLA/PCL-PLGA/Mg(OH)₂, which includes a cartilage layer, an osteochondral interface, and a bone layer. The scaffold was filled with Velvet antler polypeptides (VAP), and its characterization was assessed using compression testing, XRD, FTIR, SEM, fluorescence microscopy, and EDS. In vitro investigation demonstrated that the scaffold not only supported osteogenesis but also promoted chondrogenic differentiation of fibrocartilage stem cells (FCSCs). n vivo experiments showed that the tri-phasic PLA/PCL-PLGA/Mg(OH)2-VAP scaffold together with FCSC, when transplanted to animal models, increased the recovery of osteochondral defects. Those results demonstrate the promising future of illustrated tri-phasic PLA/PCL-PLGA/Mg(OH)2-VAP scaffold loaded with FCSCs as a new bone and cartilage tissue engineering approach for osteochondral defects treatment.

    Keywords: Fibrocartilage stem cells, Velvet antler polypeptide, Tissue Engineering, Triphasic scaffold, osteochondral defects

    Received: 06 Jul 2024; Accepted: 06 Sep 2024.

    Copyright: © 2024 Renyi, Tao, Ma, Peishen, Chaofeng, Yuchen, Changyu, jinjun and Yanhua. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Deng Peishen, Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
    Liu Chaofeng, Second Clinic, Kunming Medical University Affiliated Stomatological Hospital, Kunming, China
    Hong Yuchen, Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
    Liu Changyu, Department of Orthodontics, Affiliated Stomatology Hospital of Kunming Medical University, Kunming, Yunnan Province, China
    Tian jinjun, Yunnan Key Laboratory of Stomatology, Kunming, China
    Xu Yanhua, Yunnan Key Laboratory of Stomatology, Kunming, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.