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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Nanobiotechnology
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1455385
This article is part of the Research Topic Advancing Multidisciplinary Approaches for Combating Multidrug-Resistant Infections View all articles

Antibacterial and Antibiofilm Activity of Silver Nanoparticles Stabilized with C-Phycocyanin Against Drug-Resistant Pseudomonas aeruginosa and Staphylococcus aureus

Provisionally accepted
Zahra Chegini Zahra Chegini 1Aref Shariati Aref Shariati 2Mohammad Y. Alikhani Mohammad Y. Alikhani 1*Maliheh Safaiee Maliheh Safaiee 1*Shahin Rajaeih Shahin Rajaeih 3*Mohammad R. Arabestani Mohammad R. Arabestani 1*Mehdi Azizi Mehdi Azizi 1*
  • 1 Hamadan University of Medical Sciences, Hamedan, Iran
  • 2 Arak University of Medical Sciences, Arak, Markazi, Iran
  • 3 Iran University of Medical Sciences, Tehran, Tehran, Iran

The final, formatted version of the article will be published soon.

    Background: Biofilms are bacterial communities that can protect them against external factors, including antibiotics. In this study, silver nanoparticles (AgNPs) were formed by modifying AgNPs with C-phycocyanin (Ag-Pc) to inhibit the growth of carbapenem-resistant Pseudomonas aeruginosa (CR P. aeruginosa) and methicillin-resistant Staphylococcus aureus (MRSA) and destroy biofilm of these bacteria.The AgNPs were prepared with the green synthesis method, and Pc was used to stabilize the AgNPs. The Ag-Pc's antibacterial and antibiofilm effects were evaluated using the Microbroth dilution method and microtiter plate assay. The inhibitory effect of Ag-Pc on the expression of biofilm-related genes was evaluated by real-time PCR. Moreover, the MTT assay was used to assess the Ag-Pc toxicity.The Ag-Pc minimum inhibitory concentration (MIC) was 7.4 µg/ml for CR P. aeruginosa and MRSA. Pc did not show antibacterial effects against any of the strains. Ag-Pc suppressed biofilm formation and destroyed matured biofilm in both bacteria more efficiently than the AgNPs (P<0.05). The expression of all genes was not significantly reduced in the presence of synthesized nanoparticles. Finally, the MTT assay results did not show toxicity against a murine fibroblast cell line (L929) at MIC concentration.The present study showed the promising potential of Pc for improving the antibacterial and antibiofilm function of AgNPs and inhibiting drug-resistant bacteria. Therefore, Ag-Pc nanoparticles can be considered a promising therapeutic approach for the managing of the bacterial biofilm.

    Keywords: antimicrobial resistance, silver nanoparticles, C-phycocyanin, Biofilms, Antibio tics-resistant

    Received: 26 Jun 2024; Accepted: 07 Oct 2024.

    Copyright: © 2024 Chegini, Shariati, Alikhani, Safaiee, Rajaeih, Arabestani and Azizi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Mohammad Y. Alikhani, Hamadan University of Medical Sciences, Hamedan, Iran
    Maliheh Safaiee, Hamadan University of Medical Sciences, Hamedan, Iran
    Shahin Rajaeih, Iran University of Medical Sciences, Tehran, 14496, Tehran, Iran
    Mohammad R. Arabestani, Hamadan University of Medical Sciences, Hamedan, Iran
    Mehdi Azizi, Hamadan University of Medical Sciences, Hamedan, Iran

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