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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Bioprocess Engineering
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1447265

Establishment of the microscope incubation system and its application in evaluation of tumor treatment effects by real time live cells imaging

Provisionally accepted
Haiyang Yan Haiyang Yan 1,2Tong Wu Tong Wu 2Xinlu Li Xinlu Li 1Feng Zhengyang Feng Zhengyang 3Mingfeng GE Mingfeng GE 2*Lixing Zhang Lixing Zhang 2*Wen-Fei Dong Wen-Fei Dong 2*
  • 1 University of Science and Technology of China, Hefei, Anhui Province, China
  • 2 Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences (CAS), Suzhou, China
  • 3 Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    Long-term imaging of live cells is commonly used for the study of dynamic cell behaviors. It is crucial to keep the cell viability during the investigation of physiological and biological processes by live cell imaging. Conventional incubators that providing stable temperature, carbon dioxide (CO2) concentration, and humidity are often incompatible with most imaging tools. Available commercial or custommade stage-top incubators are bulky or unable to provide constant environmental conditions during long time culture. In this study, we reported the development of the microscope incubation system (MIS) that can be easily adapted to any inverted

    Keywords: Microscope incubation system, Long-term imaging, PID Algorithm, Organoids culture, Live cell microscope

    Received: 11 Jun 2024; Accepted: 22 Jul 2024.

    Copyright: © 2024 Yan, Wu, Li, Zhengyang, GE, Zhang and Dong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Mingfeng GE, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences (CAS), Suzhou, China
    Lixing Zhang, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences (CAS), Suzhou, China
    Wen-Fei Dong, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences (CAS), Suzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.