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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Biosensors and Biomolecular Electronics
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1444846
This article is part of the Research Topic FEAST of biosensors: Food, environmental, and agricultural sensing techniques (FEAST) View all 4 articles

Based on mutated aptamer-smartphone colorimetric detection of metronidazole in milk

Provisionally accepted
Sicheng Zhang Sicheng Zhang 1Yadi Qin Yadi Qin 1Jie Yuan Jie Yuan 2Yu Wang Yu Wang 1Jun Yao Jun Yao 1*Minwei Zhang Minwei Zhang 3*
  • 1 Xinjiang Medical University, Ürümqi, China
  • 2 School of Pharmacy, Xinjiang Second Medical College, WULUMUQI, China
  • 3 Xinjiang University, Urumqi, Xinjiang Uyghur Region, China

The final, formatted version of the article will be published soon.

    Excessive residue of metronidazole (MNZ) in food is harmful to the human body. There is an urgent demand to develop a portable tool for MNZ detection on-site.In this study, fifteen aptamers were prepared through targeted base mutation. Apt1-3 with the highest enrichment was chosen for further study. Its affinity was characterized by molecular docking simulation, AuNPs colorimetric assay, graphene oxide (GO) fluorescence assay, and exonuclease assay. Kd was determined by GO fluorescence assay (Kd: 92.60 ± 25.59 nM). Its specificity was also characterized by an exonuclease assay. A novel aptasensor was constructed by using the newly identified aptamer combined with the smartphone dark box. The principle of color change is caused by the aggregation state of AuNPs. Smartphones act as reading instruments. The detection can be completed in just a few seconds without the aid of instruments, achieving a detection limit of 0.15 nmol/mL and a range of 6.7-44.4 nmol/mL (R 2 = 0.9810). Therefore, the constructed smartphone colorimetric sensor based on mutant aptamers has important applications in food detection.

    Keywords: aptamer, Colorimetric, Metronidazole, Base mutation, smartphone

    Received: 06 Jun 2024; Accepted: 19 Jul 2024.

    Copyright: © 2024 Zhang, Qin, Yuan, Wang, Yao and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jun Yao, Xinjiang Medical University, Ürümqi, China
    Minwei Zhang, Xinjiang University, Urumqi, 830046, Xinjiang Uyghur Region, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.