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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Tissue Engineering and Regenerative Medicine
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1441547
This article is part of the Research Topic Recent Advances in Platelet-Concentrate Therapy in Regenerative Medicine View all 6 articles

Release kinetics of growth factors loaded into β-TCP ceramics in an in vitro model

Provisionally accepted
Marco Waldmann Marco Waldmann 1Marc Bohner Marc Bohner 2Anna Baghnavi Anna Baghnavi 1Bianca Riedel Bianca Riedel 1Michael Seidenstuecker Michael Seidenstuecker 1*
  • 1 G.E.R.N. Center of Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg i. Br., Germany
  • 2 RMS Foundation, Bettlach, Solothurn, Switzerland

The final, formatted version of the article will be published soon.

    β-TCP ceramics are bone replacement materials that have recently been tested as a drug delivery system which can potentially be applied to endogenous substances like growth factors found in blood platelets to facilitate positive attributes. In this work, we used flow chamber loading to load β-TCP dowels with blood suspensions of platelet-rich plasma, platelet-poor plasma or buffy coat character. PRP and BC platelet counts were adjusted to the same level by dilution. Concentrations of TGF-β1, PDGF-AB and IGF-1 from dowel surrounding culture medium were subsequently determined by means of ELISA over a time of 5 days. Influence of alginate was additionally tested to modify release. Concentrations of TGF-β1 and PDGF-AB increased and conclusively showed a release from platelets in PRP and BC compared to PPP.Alginate coating reduced PDGF-AB release but did not reduce TGF-β1 and instead even increased TGF-β1 in BC samples. IGF-1 concentrations were highest in PPP, suggesting circulating levels rather than platelet release as the driving factor. Alginate samples tended to have lower IGF-1 concentrations but could not be shown to be significant.Conclusively, the release of growth factors from different blood suspensions could be successfully demonstrated for β-TCP as a drug delivery system with release patterns that correspond to PRP activation after Ca 2+ triggered activation. The release pattern was partially modified by alginate coating.

    Keywords: β-tcp, PRP, ceramic, growth factor, TGF-beta, IGF-1, PDGF-AB

    Received: 31 May 2024; Accepted: 02 Sep 2024.

    Copyright: © 2024 Waldmann, Bohner, Baghnavi, Riedel and Seidenstuecker. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Michael Seidenstuecker, G.E.R.N. Center of Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Freiburg i. Br., Germany

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.