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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Biomaterials
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1425450

Safety of Electrochemical decontamination of titanium on Preosteoblasts: an in vitro Study

Provisionally accepted
Wenji Cai Wenji Cai 1Min Wang Min Wang 2,3Amir E. Hadad Amir E. Hadad 1Yuli Zhang Yuli Zhang 1Samar Shurbaji Samar Shurbaji 4Gheyath K. Nasrallah Gheyath K. Nasrallah 5Simon D. Tran Simon D. Tran 1Mariano Sanz Mariano Sanz 6Sasha Omanovic Sasha Omanovic 7Faleh Tamimi Faleh Tamimi 4*
  • 1 Faculty of Dental Medicine and Oral Health Sciences, McGill University, Montreal, Quebec, Canada
  • 2 State Key Laboratory Breeding Base of Basic Science of Stomatology, Ministry of Education, Hospital of Stomatology, Faculty of Medical Sciences, Wuhan University, Wuhan, China
  • 3 Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan, China
  • 4 College of Dental Medicine, Qatar University, Doha, Qatar
  • 5 Department of Biomedical Sciences, College of Health Sciences, Qatar University, Doha, Qatar
  • 6 Faculty of Dentistry, Complutense University of Madrid, Madrid, Spain
  • 7 Department of Chemical Engineering, Faculty of Engineering, McGill University, Montreal, Canada

The final, formatted version of the article will be published soon.

    The aim of this study was to investigate the impact of electrochemical treatments designed to decontaminate titanium on the viability of tissue cells attached to pure titanium substrates and determine safety limits for this type of treatment.Methods: Pre-osteoblast cells (pOB) were cultured and seeded onto titanium discs. The cellseeded discs were then exposed to a range of fixed direct electrical potentials (-6V to 6V) or fixed direct electrical currents (-12.5mA, -25mA, or -50mA) using a three-electrode system connected to a potentiostat. Cell viability was assessed using live/dead assay and fluorescence microscopy.Results: Exposure of cells to strong negative potentials caused cell detachment, while exposure to positive ones led to cell death on the cpTi surfaces. However, cellular viability was preserved when the electrical potentials were kept between -3 to +3 V. Cells retained 80% viability when subjected to -12.5 mA currents with an initial pOB cell count of 5x10 4 . However, when the initial cell count was elevated to 1x10 5 , the cells demonstrated the ability to withstand an even greater current (-25mA) while preserving their vitality at the same level.Conclusion: Electrical potentials can harm cells surrounding dental implants. However, this damage can be minimized by keeping the potential within a safety limit.

    Keywords: Pre-osteoblast cells, electrochemical treatment, Titanium, implants, voltage, current, Osteointegration

    Received: 29 Apr 2024; Accepted: 23 Sep 2024.

    Copyright: © 2024 Cai, Wang, Hadad, Zhang, Shurbaji, Nasrallah, Tran, Sanz, Omanovic and Tamimi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Faleh Tamimi, College of Dental Medicine, Qatar University, Doha, Qatar

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