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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Biosensors and Biomolecular Electronics
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1417742
This article is part of the Research Topic Advances in Implantable Devices for Personalized Biomedicine View all articles

Honeycomb-like Biomimetic Scaffold by Functionalized Antibacterial Hydrogel and Biodegradable Porous Mg Alloy for Osteochondral Regeneration

Provisionally accepted
Yongqiang Zhang Yongqiang Zhang 1,2,3Qiangsheng Dong Qiangsheng Dong 4*Xiao Zhao Xiao Zhao 1,2,3*Yuzhi Sun Yuzhi Sun 1,2,3*Xin Lin Xin Lin 5*Xin Zhang Xin Zhang 1,2,3*Tianming Wang Tianming Wang 1,2,3Tianxiao Yang Tianxiao Yang 1,2,3*Xiao Jiang Xiao Jiang 1,2,3*Jiaxiang Li Jiaxiang Li 1,2,3*Zhicheng Cao Zhicheng Cao 1,2,3*Tingwen Cai Tingwen Cai 1,2,3*Wanshun Liu Wanshun Liu 1,2,3*Hongjing Zhang Hongjing Zhang 1,2,3*Jing Bai Jing Bai 6,7Qingqiang Yao Qingqiang Yao 1,2,3*
  • 1 Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
  • 2 Key Lab of Additive Manufacturing Technology, Institute of Digital Medicine, Nanjing Medical University, Nanjing, Liaoning Province, China
  • 3 Research Center of Digital Medicine and 3D Printing Technology of Jiangsu Province, Nanjing, China
  • 4 School of Materials Science and Engineering, Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology, Nanjing Institute of Technology, Nanjing, China
  • 5 Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
  • 6 School of Materials Science and Engineering, Southeast University, Nanjing, Jiangsu Province, China
  • 7 Nanjing Institute of Biomaterials and Medical Devices, Southeast University, Nanjing, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    Osteochondral repair is a significant challenge due to its unique pathological mechanisms and complex repair processes, especially in bacterial tissue conditions caused by open injury, infection and surgical contamination. The work introduced a biomimetic honeycomb-like scaffold (Zn-AlgMA@Mg) for osteochondral repair, composed of a dicalcium phosphate dihydrate (DCPD) coated porous magnesium scaffold (DCPD Mg) embedded within a dual crosslinked sodium alginate hydrogel (Zn-AlgMA), which synergistically exert antibacterial and osteochondral integrated repair properties. The DCPD Mg displayed a uniform porous structure and superior mechanical properties, and the Zn-AlgMA exhibited consistent microstructural features and enhanced hydrophilicity. The Zn-AlgMA@Mg scaffold provided controlled release of magnesium and zinc ions for cell proliferation and vitality, exhibiting significant osteogenic and chondrogenic properties as well as antibacterial efficacy in vitro. Proteomic analysis revealed the mechanism of osteochondral integrated repair from this scaffold. Micro-CT evaluation from three-dimensional reconstruction, quantitative analysis, and histology (including hematoxylin-eosin, Safranin-O, and Masson's trichrome staining) demonstrated that osteochondral integration was successful in the rabbit full-thickness osteochondral defect model. Therefore, this biomimetic honeycomb-like scaffold (Zn-AlgMA@Mg) provided a promising biomaterial for achieving integrated osteochondral regeneration.

    Keywords: osteochondral defects, Biomimetic scaffold, honeycomb, magnesium alloy, Sodium alginate hydrogel, Zinc ions

    Received: 15 Apr 2024; Accepted: 20 Jun 2024.

    Copyright: © 2024 Zhang, Dong, Zhao, Sun, Lin, Zhang, Wang, Yang, Jiang, Li, Cao, Cai, Liu, Zhang, Bai and Yao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Qiangsheng Dong, School of Materials Science and Engineering, Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology, Nanjing Institute of Technology, Nanjing, China
    Xiao Zhao, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Yuzhi Sun, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Xin Lin, Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Xin Zhang, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Tianxiao Yang, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Xiao Jiang, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Jiaxiang Li, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Zhicheng Cao, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Tingwen Cai, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Wanshun Liu, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Hongjing Zhang, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
    Qingqiang Yao, Department of Orthopaedics, Nanjing First Hospital, Nanjing Medical University, Nanjing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.