AUTHOR=Ma Cuidie , Xu Zhihui , Hao Kun , Fan Lingling , Du Wenqian , Gao Zhan , Wang Chong , Zhang Zheng , Li Ningxia , Li Qi , Gao Qi , Yu Changyuan 

TITLE=Rapid isolation method for extracellular vesicles based on Fe3O4@ZrO2

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=12

YEAR=2024

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2024.1399689

DOI=10.3389/fbioe.2024.1399689

ISSN=2296-4185

ABSTRACT=<p>Extracellular vesicles (EVs) are pivotal in intercellular communication, disease mechanisms. Despite numerous methods for EVs isolation, challenges persist in yield, purity, reproducibility, cost, time, and automation. We introduce a EVs isolation technique using Fe<sub>3</sub>O<sub>4</sub>@ZrO<sub>2</sub> beads, leveraging ZrO<sub>2</sub>-phosphate interaction. The results indicated that EVs were efficiently separated from large volumes of samples in 30 minutes without preconcentration. Our method demonstrated capture efficiency (74%–78%) compared to ultracentrifugation, purity (97%), and reproducibility (0.3%–0.5%), with excellent linearity (R<sup>2</sup> &gt; 0.99). EVs from urine samples showed altered expression of miRNAs. The logistic regression model achieved an AUC of 0.961, sensitivity of 0.92, and specificity of 0.94. With potential for automation, this magnetic bead-based method holds promise for clinical applications, offering an efficient and reliable tool for EVs research and clinical studies.</p>