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ORIGINAL RESEARCH article

Front. Bioeng. Biotechnol.
Sec. Biomaterials
Volume 12 - 2024 | doi: 10.3389/fbioe.2024.1355493

Zinc and Chitosan-Enhanced β-tricalcium Phosphate from Calcined Fetal Bovine Bone for Mandible Reconstruction

Provisionally accepted
Jianye Zhou Jianye Zhou 1Rui Ma Rui Ma 1Wen Shi Wen Shi 1Shennan Lei Shennan Lei 1Xiaohui Zhang Xiaohui Zhang 1Nan Jiang Nan Jiang 2Yongsheng Lin Yongsheng Lin 1Zhiqiang Li Zhiqiang Li 1*Min Nie Min Nie 3*
  • 1 Key Laboratory of Oral Diseases of Gansu Province, Northwest University for Nationalities, Lanzhou, Gansu Province, China
  • 2 Institute of Applied Ecology, Chinese Academy of Sciences (CAS), Shenyang, Liaoning Province, China
  • 3 Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine,, Guangzhou, China

The final, formatted version of the article will be published soon.

    Background: Mandibular defects pose significant challenges in reconstructive surgery, and scaffold materials are increasingly recognized for their potential to address these challenges. Among various scaffold materials, Beta-tricalcium phosphate (β-TCP) is noted for its exceptional osteogenic properties. However, improvements in its biodegradation rate and mechanical strength are essential for optimal performance. Methods: In this study, we developed a novel β-TCP-based scaffold, CFBB, by calcining fetal bovine cancellous bone. To enhance its properties, we modified CFBB with Chitosan (CS) and Zinc (Zn), creating three additional scaffold materials: CFBB/CS, CFBB/Zn 2+ , and CFBB/Zn 2+ /CS. We conducted comprehensive assessments of their physicochemical and morphological properties, degradation rates, biocompatibility, osteogenic ability, new bone formation, and neovascularization both in vitro and in vivo.Results: Our findings revealed that all four materials were biocompatible and safe for use. The modifications with CS and Zn 2+ significantly improved the mechanical strength, osteogenic, and angiogenic properties of CFBB, while concurrently decelerating its resorption rate. Among the tested materials, CFBB/Zn 2+ /CS demonstrated superior performance in promoting bone regeneration and vascularization, making it a particularly promising candidate for mandibular reconstruction.The CFBB/Zn 2+ /CS scaffold material, with its enhanced mechanical, osteogenic, and angiogenic properties, and a controlled resorption rate, emerges as a highly effective alternative for the repair of oral mandible defects. This study underscores the potential of combining multiple bioactive agents in scaffold materials to improve their functionality for specific clinical applications in bone tissue engineering.

    Keywords: Chitosan, Zinc, Calcined fetal bovine bone, Beta-tricalcium phosphate, Biocompatibility, physicochemical properties, Osteogenic ability

    Received: 14 Dec 2023; Accepted: 17 Sep 2024.

    Copyright: © 2024 Zhou, Ma, Shi, Lei, Zhang, Jiang, Lin, Li and Nie. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Zhiqiang Li, Key Laboratory of Oral Diseases of Gansu Province, Northwest University for Nationalities, Lanzhou, Gansu Province, China
    Min Nie, Department of Periodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine,, Guangzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.