AUTHOR=Wang Qianru , Kim Hyungseok , Halvorsen Tiffany M. , Chen Sijie , Hayes Christopher S. , Buie Cullen R. TITLE=Leveraging microfluidic dielectrophoresis to distinguish compositional variations of lipopolysaccharide in E. coli JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=11 YEAR=2023 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.991784 DOI=10.3389/fbioe.2023.991784 ISSN=2296-4185 ABSTRACT=
Lipopolysaccharide (LPS) is the unique feature that composes the outer leaflet of the Gram-negative bacterial cell envelope. Variations in LPS structures affect a number of physiological processes, including outer membrane permeability, antimicrobial resistance, recognition by the host immune system, biofilm formation, and interbacterial competition. Rapid characterization of LPS properties is crucial for studying the relationship between these LPS structural changes and bacterial physiology. However, current assessments of LPS structures require LPS extraction and purification followed by cumbersome proteomic analysis. This paper demonstrates one of the first high-throughput and non-invasive strategies to directly distinguish