AUTHOR=Ahmad Shahbaz , Ahmad Shujaat , Xu Qianqian , Khan Idrees , Cao Xiaoyu , Yang Ruimin , Yan Hai 

TITLE=Green synthesis of gold and silver nanoparticles using crude extract of Aconitum violaceum and evaluation of their antibacterial, antioxidant and photocatalytic activities

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2024

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1320739

DOI=10.3389/fbioe.2023.1320739

ISSN=2296-4185

ABSTRACT=<p>Green synthesis of metal nanoparticles (NPs) has received extensive attention over other conventional approaches due to their non-toxic nature and more biocompatibility. Herein we report gold and silver NPs (AuNPs@AV and AgNPs@AV) prepared by employing a green approach using crude extract of <italic>Aconitum violaceum</italic> Jacquem. ex Stapf. The synthesized NPs were characterized using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX), X-ray Diffraction (XRD), UV/Visible spectroscopy, Fourier Transform Infrared (FTIR), X-ray Photoelectron Spectroscopy (XPS), and Zeta Potential. Morphological analysis showed spherical and triangular shapes of the NPs with average size of &lt;100 nm. The AuNPs@AV and AgNPs@AV exhibited effective antibacterial activities, with minimum inhibitory concentrations (MICs) of 95 and 70 μg/mL against <italic>Lactobacillus acidophilus</italic> (<italic>L. acidophilus</italic>) and 90 and 65 μg/mL against <italic>Escherichia coli</italic> (<italic>E. coli</italic>), respectively. Strong antioxidant effect of AuNPs@AV and AgNPs@AV were reported against DPPH radical and PTIO within range of IC<sub>50</sub> values; 161–80 μg/ml as compared to the standard (23–11 μg/mL) respectively. Moreover, the AuNPs@AV and AgNPs@AV showed efficient photocatalytic activity and degraded 89.88% and 93.7% methylene blue (MB) dye under UV light, respectively.</p>