AUTHOR=Lin Jiasheng , Li Sanhua , Ye Benchen , Zheng Weigang , Wang Huihui , Liu Ying , Wang Dong , Wu Zaihui , Dong Wen-Fei , Zan Minghui 

TITLE=A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1308725

DOI=10.3389/fbioe.2023.1308725

ISSN=2296-4185

ABSTRACT=<p><bold>Introduction:</bold> Pregnanediol-3-glucuronide (PdG), as the main metabolite of progesterone in urine, plays a significant role in the prediction of ovulation, threatened abortion, and menstrual cycle maintenance.</p><p><bold>Methods:</bold> To achieve a rapid and sensitive assay, we have designed a competitive model-based time-resolved fluorescence microsphere-lateral flow immunochromatography (TRFM-LFIA) strip.</p><p><bold>Results:</bold> The optimized TRFM-LFIA strip exhibited a wonderful response to PdG over the range of 30–2,000 ng/mL, the corresponding limit of detection (LOD) was calculated as low as 8.39 ng/mL. More importantly, the TRFM-LFIA strip was innovatively used for the quantitative detection of PdG in urine sample, and excellent recovery results were also obtained, ranging from 97.39% to 112.64%.</p><p><bold>Discussion:</bold> The TRFMLFIA strip possessed robust sensitivity and selectivity in the determination of PdG, indicating the great potential of being powerful tools in the biomedical and diagnosis region.</p>