AUTHOR=Zhao Pengxuan , Zhong Yeteng , Pan Pengcheng , Zhang Shasha , Tian Yu , Zhang Jun , Yi Guohui , Zhao Zhendong , Wu Tiantian 

TITLE=DNA self-assembly nanoflower reverse P-glycoprotein mediated drug resistance in chronic myelogenous leukemia therapy

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1265199

DOI=10.3389/fbioe.2023.1265199

ISSN=2296-4185

ABSTRACT=<p><bold>Introduction:</bold> Chronic myelogenous leukemia (CML) is a clonal myeloproliferative disorder caused by the BCR-ABL chimeric tyrosine kinase. Vincristine (VCR) is widely used in leukemia therapy but is hindered by multidrug resistance (MDR).</p><p><bold>Methods:</bold> We prepared DNA nanoflower via self-assembly for the delivery of VCR and P-glycoprotein small interfering RNA (P-gp siRNA).</p><p><bold>Results and Discussion:</bold> The as-prepared nanoflower had a floriform shape with high loading efficiency of VCR (80%). Furthermore, the nanoflower could deliver VCR and P-gp siRNA into MDR CML cells and induce potent cytotoxicity both <italic>in vitro</italic> and <italic>in vivo</italic>, thus overcoming MDR of CML. Overall, this nanoflower is a promising tool for resistant CML therapy.</p>