AUTHOR=Ren Xinru , Wei Yue , Zhao Honglu , Shao Juanjuan , Zeng Fanli , Wang Zhen , Li Li 

TITLE=A comprehensive review and comparison of L-tryptophan biosynthesis in Saccharomyces cerevisiae and Escherichia coli

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1261832

DOI=10.3389/fbioe.2023.1261832

ISSN=2296-4185

ABSTRACT=<p>L-tryptophan and its derivatives are widely used in the chemical, pharmaceutical, food, and feed industries. Microbial fermentation is the most commonly used method to produce L-tryptophan, which calls for an effective cell factory. The mechanism of L-tryptophan biosynthesis in <italic>Escherichia coli</italic>, the widely used producer of L-tryptophan, is well understood. <italic>Saccharomyces cerevisiae</italic> also plays a significant role in the industrial production of biochemicals. Because of its robustness and safety, <italic>S. cerevisiae</italic> is favored for producing pharmaceuticals and food-grade biochemicals. However, the biosynthesis of L-tryptophan in <italic>S. cerevisiae</italic> has been rarely summarized. The synthetic pathways and engineering strategies of L-tryptophan in <italic>E. coli</italic> and <italic>S. cerevisiae</italic> have been reviewed and compared in this review. Furthermore, the information presented in this review pertains to the existing understanding of how L-tryptophan affects <italic>S. cerevisiae’s</italic> stress fitness, which could aid in developing a novel plan to produce more resilient industrial yeast and <italic>E. coli</italic> cell factories.</p>