AUTHOR=Wei Yanwei , Zhang Shuyue , Guan Guikun , Wan Ziran , Wang Ruiming , Li Piwu , Liu Yu , Wang Junqing , Jiao Guanhua , Wang Hao , Sun Chuying 

TITLE=A specific and rapid method for detecting Bacillus and Acinetobacter species in Daqu

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1261563

DOI=10.3389/fbioe.2023.1261563

ISSN=2296-4185

ABSTRACT=<p>Daqu is a spontaneous, solid-state cereal fermentation product used for saccharification and as a starter culture for Chinese Baijiu production. <italic>Bacillus</italic> and <italic>Acinetobacter</italic>, two dominant microbial genera in Daqu, produce enzymes and organic acids that influence the Daqu quality. However, there are no rapid analytical methods for detecting <italic>Bacillus</italic> and <italic>Acinetobacter</italic>. We designed primers specific to the genera <italic>Bacillus</italic> and <italic>Acinetobacter</italic> to perform genetic comparisons using the 16 S rRNA. After amplification of polymerase chain reaction using specific primers, high-throughput sequencing was performed to detect strains of <italic>Bacillus</italic> and <italic>Acinetobacter</italic>. The results showed that the effective amplification rates for <italic>Bacillus</italic> and <italic>Acinetobacter</italic> in Daqu were 86.92% and 79.75%, respectively. Thus, we have devised and assessed a method to accurately identify the species associated with <italic>Bacillus</italic> and <italic>Acinetobacter</italic> in Daqu, which can also hold significance for bacterial typing and identification.</p>