AUTHOR=Won Dong-Sung , Park Yubeen , Zeng Chu Hui , Ryu Dae Sung , Kim Ji Won , Kang Jeon Min , Kim Song Hee , Kim Hyung-Sik , Lee Sang Soo , Park Jung-Hoon 

TITLE=Radiofrequency ablation via an implanted self-expandable metallic stent to treat in-stent restenosis in a rat gastric outlet obstruction model

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1244569

DOI=10.3389/fbioe.2023.1244569

ISSN=2296-4185

ABSTRACT=<p><bold>Background:</bold> In-stent restenosis caused by tissue hyperplasia and tumor growth through the wire meshes of an implanted self-expandable metallic stent (SEMS) remains an unresolved obstacle. This study aimed to investigate the safety and efficacy of SEMS-mediated radiofrequency ablation (RFA) for treating stent-induced tissue hyperplasia in a rat gastric outlet obstruction model.</p><p><bold>Methods:</bold> The ablation zone was investigated using extracted porcine liver according to the ablation time. The optimal RFA parameters were evaluated in the dissected rat gastric outlet. We allocated 40 male rats to four groups of 10 rats as follows: group A, SEMS placement only; group B, SEMS-mediated RFA at 4 weeks; group C, SEMS-mediated RFA at 4 weeks and housed until 8 weeks; and group D, SEMS-mediated RFA at 4 and 8 weeks. Endoscopy and fluoroscopy for <italic>in vivo</italic> imaging and histological and immunohistochemical analysis were performed to compare experimental groups.</p><p><bold>Results:</bold> Stent placement and SEMS-mediated RFA with an optimized RFA parameter were technically successful in all groups. Granulation tissue formation-related variables were significantly higher in group A than in groups B–D (all <italic>p</italic> &lt; 0.05). Endoscopic and histological findings confirmed that the degrees of stent-induced tissue hyperplasia in group D were significantly lower than in groups B and C (all <italic>p</italic> &lt; 0.05). Hsp70 and TUNEL expressions were significantly higher in groups B–D than in group A (all <italic>p</italic> &lt; 0.001).</p><p><bold>Conclusion:</bold> The implanted SEMS-mediated RFA successfully managed stent-induced tissue hyperplasia, and repeated or periodic RFA seems to be more effective in treating in-stent restenosis in a rat gastric outlet obstruction model.</p>