AUTHOR=Pisani Silvia , Mauri Valeria , Negrello Erika , Mauramati Simone , Alaimo Gianluca , Auricchio Ferdinando , Benazzo Marco , Dorati Rossella , Genta Ida , Conti Bice , Ferretti Virginia Valeria , De Silvestri Annalisa , Pietrabissa Andrea , Marconi Stefania 

TITLE=Assessment of different manufacturing techniques for the production of bioartificial scaffolds as soft organ transplant substitutes

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1186351

DOI=10.3389/fbioe.2023.1186351

ISSN=2296-4185

ABSTRACT=<p><bold>Introduction:</bold> The problem of organs’ shortage for transplantation is widely known: different manufacturing techniques such as Solvent casting, Electrospinning and 3D Printing were considered to produce bioartificial scaffolds for tissue engineering purposes and possible transplantation substitutes. The advantages of manufacturing techniques’ combination to develop hybrid scaffolds with increased performing properties was also evaluated.</p><p><bold>Methods:</bold> Scaffolds were produced using poly-L-lactide-co-caprolactone (PLA-PCL) copolymer and characterized for their morphological, biological, and mechanical features.</p><p><bold>Results:</bold> Hybrid scaffolds showed the best properties in terms of viability (&gt;100%) and cell adhesion. Furthermore, their mechanical properties were found to be comparable with the reference values for soft tissues (range 1–10 MPa).</p><p><bold>Discussion:</bold> The created hybrid scaffolds pave the way for the future development of more complex systems capable of supporting, from a morphological, mechanical, and biological standpoint, the physiological needs of the tissues/organs to be transplanted.</p>