AUTHOR=Panayotov Ivan V. , Végh Attila G. , Martin Marta , Vladimirov Boyan , Larroque Christian , Gergely Csilla , Cuisinier Frédéric J. G. , Estephan Elias 

TITLE=Improving dental epithelial junction on dental implants with bioengineered peptides

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1165853

DOI=10.3389/fbioe.2023.1165853

ISSN=2296-4185

ABSTRACT=<p><bold>Introduction:</bold> The functionalization of titanium (Ti) and titanium alloys (Ti6Al4V) implant surfaces via material-specific peptides influence host/biomaterial interaction. The impact of using peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported.</p><p><bold>Results:</bold> The metal binding peptides (MBP-1, MBP-2) SVSVGMKPSPRP and WDPPTLKRPVSP were selected via phage display and combined with laminin-5 or E-cadherin epithelial cell specific peptides (CSP-1, CSP-2) to engineer four metal-cell specific peptides (MCSPs). Single-cell force spectroscopy and cell adhesion experiments were performed to select the most promising candidate. <italic>In vivo</italic> tests using the dental implant for rats showed that the selected bi functional peptide not only enabled stable cell adhesion on the trans-gingival part of the dental implant but also arrested the unwanted apical migration of epithelial cells.</p><p><bold>Conclusion:</bold> The results demonstrated the outstanding performance of the bioengineered peptide in improving epithelial adhesion to Ti based implants and pointed towards promising new opportunities for applications in clinical practice.</p>