AUTHOR=Song Mingzhe , Yi Liang , Tang Zhenjie , Xie Xinlong , Liu Yuhong , Qi XiaoKe , Jiang Zhenlin , Chen ZeGuo , Chen Chunyang , Wu QiYing , Wu ZhongShi TITLE=Preliminary study of improving immune tolerance in vivo of bioprosthetic heart valves through a novel antigenic removal method JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=11 YEAR=2023 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1141247 DOI=10.3389/fbioe.2023.1141247 ISSN=2296-4185 ABSTRACT=

The durability of bioprosthetic heart valves is always compromised by the inherent antigenicity of biomaterials. Decellularization has been a promising approach to reducing the immunogenicity of biological valves. However, current methods are insufficient in eliminating all immunogenicity from the biomaterials, necessitating the exploration of novel techniques. In this study, we investigated using a novel detergent, fatty alcohol polyoxyethylene ether sodium sulfate (AES), to remove antigens from bovine pericardium. Our results demonstrated that AES treatment achieved a higher pericardial antigen removal rate than traditional detergent treatments while preserving the mechanical properties and biocompatibility of the biomaterials. Moreover, we observed excellent immune tolerance in the in vivo rat model. Overall, our findings suggest that AES treatment is a promising method for preparing biological valves with ideal clinical application prospects.