AUTHOR=Ren Juan , Rao Jianping , Wang He , He Wenjing , Feng Jinnan , Wei Danni , Zhao Bin , Wang Xing , Bian Wei 

TITLE=Synergistic remineralization of enamel white spot lesions using mesoporous bioactive glasses loaded with amorphous calcium phosphate

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2023.1109195

DOI=10.3389/fbioe.2023.1109195

ISSN=2296-4185

ABSTRACT=<p><bold>Objectives:</bold> The purpose of this study was to create a new delivery system that can synergistically remineralize enamel white spot lesions (WSLs).</p><p><bold>Materials and methods:</bold> The delivery system (PAA-ACP@aMBG) was prepared by using aminated mesoporous bioactive glasses (aMBG) as the carrier loaded with polyacrylic-stabilized amorphous calcium phosphate (PAA-ACP). The materials were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), inductively coupled plasma–optical emission spectrometry (ICP–OES), and so on. Forty-eight artificial WSLs enamel samples were randomized to four groups: artificial saliva (negative control, NC), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), PAA-ACP@aMBG, and MBG. The effects of demineralization and remineralization of the enamel surface were compared by means of surface microhardness (SMH) measurements, surface color change measurements, fluorescence microscopy (FM), X-ray diffraction (XRD) analysis and scanning electron microscopy (SEM).</p><p><bold>Results:</bold> There was no significant difference in the surface microhardness recovery rate (SMHRR) or color recovery rate (CRR) among the CPP-ACP group, PAA-ACP@aMBG group and MBG group (P&gt;0.05), but these values were significantly higher than those in the NC group (<italic>p</italic> &lt; 0.01). FM demonstrated that the remineralization depth in the PAA-ACP@aMBG group was significantly greater than that of the remaining three groups (<italic>p</italic> &lt; 0.01). SEM analysis indicated that the enamel demineralization marks in the PAA-ACP@aMBG group, CPP-ACP group, and MBG group were obscured by mineral deposition.</p><p><bold>Conclusions:</bold> PAA-ACP@aMBG showed good mineralization properties, implying its great potential for clinical application.</p>