AUTHOR=Ma Xiaowei , Xu Jingsong , Zhou Fei , Ye Jing , Yang Donglei , Wang Hua , Wang Pengfei , Li Min TITLE=Recent advances in PCR-free nucleic acid detection for SARS-COV-2 JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.999358 DOI=10.3389/fbioe.2022.999358 ISSN=2296-4185 ABSTRACT=

As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold standard method for SARS-COV-2 detection, which, however, generally requires sophisticated facilities and laboratory space, and is time consuming. This review presents recent advances in PCR-free nucleic acid detection methods for SARS-CoV-2, including emerging methods of isothermal amplification, nucleic acid enzymes, electrochemistry and CRISPR.