AUTHOR=Qin Yadi , Qin Yanan , Bubiajiaer Hayilati , Chen Fengxia , Yao Jun , Zhang Minwei 

TITLE=Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.994711

DOI=10.3389/fbioe.2022.994711

ISSN=2296-4185

ABSTRACT=Various biosensors based on aptamers are currently the most popular rapid detection approaches, but the performance of these sensors is closely related to the affinity of aptamers. In this work, a strategy for constructed high-affinity aptamer was proposed. By truncating the bases flanking the 59nt dexamethasone (DEX) original aptamer sequence and then introducing base mutations, the 33nt aptamer Apt-M13 was obtained. The dissociation constant (Kd) was determined to be 200 nM by Graphene oxide (GO)-based fluorometry. Finally, Apt-M13 was used for a label-free colorimetric aptamer sensor based on gold nanoparticles, the LOD was 3.2-fold lower than the original aptamer described in previous works. The anti-interference ability of DEX analogs is also further improved. Therefore, the proposed aptamer optimization method is also expected to become a general strategy for various aptamer sequences.