AUTHOR=Tsapou Evangelia A. , Ntourtoglou George , Drosou Fotini , Tataridis Panagiotis , Lalas Stavros , Dourtoglou Vassilis 

TITLE=Pulsed electric field: A “green” extraction technology for biomolecular products from glycerol with fermentation of non-Saccharomyces yeasts

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.964174

DOI=10.3389/fbioe.2022.964174

ISSN=2296-4185

ABSTRACT=<p>Glycerol is the main organic by-product of the biodiesel industry and it can be a source of carbon for fermentations or a substrate for biotransformations. This work investigates a process that uses pulsed electric field (PEF) to enhance polyol and propanediols extraction from a glycerol/glucose fermentation broth. Three different commercial, non-<italic>Saccharomyces</italic> strains, <italic>Torulaspora delbrueckii</italic> Prelude (Hansen), <italic>Torulaspora delbrueckii</italic> Biodiva 291 (Lallemand) and <italic>Metschnikowia pulcherrima</italic> (Lallemand) were studied. The results revealed that PEF had a positive impact on the extraction of polyols ranging from 12 to 191%, independently of fermentation conditions. <italic>Torulaspora delbrueckii</italic> Biodiva 291 (Lallemand) was found to be more efficient at pH 7.1. An optimized chromatography-based method for the qualitative and quantitative determination of the formed products evaluated. The experiments were carried out either in flasks or in a bioreactor.</p>