AUTHOR=Silini Antonietta R. , Ramuta Taja Železnik , Pires Ana Salomé , Banerjee Asmita , Dubus Marie , Gindraux Florelle , Kerdjoudj Halima , Maciulatis Justinas , Weidinger Adelheid , Wolbank Susanne , Eissner Günther , Giebel Bernd , Pozzobon Michela , Parolini Ornella , Kreft Mateja Erdani TITLE=Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.958669 DOI=10.3389/fbioe.2022.958669 ISSN=2296-4185 ABSTRACT=

Perinatal derivatives or PnDs refer to tissues, cells and secretomes from perinatal, or birth-associated tissues. In the past 2 decades PnDs have been highly investigated for their multimodal mechanisms of action that have been exploited in various disease settings, including in different cancers and infections. Indeed, there is growing evidence that PnDs possess anticancer and antimicrobial activities, but an urgent issue that needs to be addressed is the reproducible evaluation of efficacy, both in vitro and in vivo. Herein we present the most commonly used functional assays for the assessment of antitumor and antimicrobial properties of PnDs, and we discuss their advantages and disadvantages in assessing the functionality. This review is part of a quadrinomial series on functional assays for the validation of PnDs spanning biological functions such as immunomodulation, anticancer and antimicrobial, wound healing, and regeneration.