AUTHOR=Liu Xina , Zhu Meiqing , Xu Chenyang , Fan Fugang , Chen Panpan , Wang Yi , Li Dongyang 

TITLE=An ICT-Based Coumarin Fluorescent Probe for the Detection of Hydrazine and Its Application in Environmental Water Samples and Organisms

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.937489

DOI=10.3389/fbioe.2022.937489

ISSN=2296-4185

ABSTRACT=<p>As an inorganic small molecule pollutant, the toxicity and potential carcinogenicity of hydrazine (N<sub>2</sub>H<sub>4</sub>) are of increasing concern. In this work, A water-soluble fluorescent probe (OCYB) based on the intramolecular charge transfer (ICT) mechanism for the detection of hydrazine was designed and synthesized. Taking the advantage of 4-bromobutyryl as the recognition group, the high selectivity of OCYB to N<sub>2</sub>H<sub>4</sub> was confirmed by steady-state fluorescence spectroscopy. The limit of detection (LOD) was calculated to be 78 nM in the DMSO-HEPES (pH 7.4) system. The detection mechanism was verified by NMR, HRMS and density functional theory (DFT) calculations. In addition, OCYB exhibits strong anti-interference ability and an “Off-On” fluorescence enhancement effect. Importantly, OCYB can be used to effectively monitor the fluorescence distribution of N<sub>2</sub>H<sub>4</sub> in environmental water samples and organisms.</p>