AUTHOR=Yan Huiling , Zhang Xinxin , Li Xiang , Wang Xuelai , Li Hanxi , Zhao Qiushuang , Yin Peng , Guo Ruixue , Pei Xiaona , Hu Xiaoqing , Han Rui , Zhao Xiyang 

TITLE=Integrated Transcriptome and Metabolome Analyses Reveal the Anthocyanin Biosynthesis Pathway in AmRosea1 Overexpression 84K Poplar

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.911701

DOI=10.3389/fbioe.2022.911701

ISSN=2296-4185

ABSTRACT=<p><italic>Populus alba</italic> × <italic>Populus glandulosa</italic> (84K poplar) is model material with excellent genetic engineering resource and ornamental value. In our study, <italic>AmRosea1</italic> (<italic>Antirrhinum majus</italic>) was overexpressed in 84K poplar, and the transgenic 84K (AM) poplar with high content of anthocyanin exhibited red pigmentation leaves. The transcriptome analysis between wild type (WT) and AM showed that 170 differentially expressed genes (DEGs) (86 up-regulated and 84 down-regulated) were found, and some DEGs were involved in flavone and flavonol biosynthesis, flavonoid biosynthesis and anthocyanin biosynthesis. The metabolome analysis showed that 13 anthocyanins-related differentially accumulated metabolites (DAMs) were detected in AM. The correlation analysis between DEGs and DAMs were performed, and the results revealed that 18 DEGs, including 11 MYB genes, two <italic>BZ1</italic> genes, one <italic>FG2</italic> gene, one <italic>ANS</italic> gene, and three <italic>IF7MAT</italic> genes, were negatively or positively correlated with 13 DAMs. The phylogenetic analysis demonstrated that there was high homology between <italic>AmRosea1</italic> and <italic>PagMYB113</italic>, and MYB113 co-expressed with BZ1, ANS and DFR directly. Our results elucidated the molecular mechanism of plant color change mediated by anthocyanin biosynthesis pathway, which laid the foundation for the development and utilization of colorful woody plant.</p>