AUTHOR=Wen Yanting , Wu Dan , Zhang Jing , Jiang Shixie , Xiong Chunyan , Guo Dan , Chi Zihui , Chen Yi , Li Lun , Yang Ying , Liu Ting , Jiang Huabei 

TITLE=Evaluation of Tracheal Stenosis in Rabbits Using Multispectral Optoacoustic Tomography

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.860305

DOI=10.3389/fbioe.2022.860305

ISSN=2296-4185

ABSTRACT=<p><bold>Objective:</bold> Photoacoustic tomography (PAT) and multispectral optoacoustic tomography (MSOT) are evolving technologies that are capable of delivering real-time, high-resolution images of tissues. The purpose of this study was to evaluate the feasibility of using PAT and MSOT for detecting histology in a rabbit tracheal stenosis model.
</p><p><bold>Method:</bold> A total of 12 rabbits (9 stenosis and three control) were randomly divided into four groups (A, B, C and D). Each group consisted of three rabbits, which were staged at the first, fourth, and eighth weeks of stenosis progression, respectively. PAT/MSOT images and corresponding histology from these experimental animals were compared, for analyzing the morphologic features and quantitative tracheal measurements in different tracheal stenosis stage.</p><p><bold>Result:</bold> Both the PAT images and corresponding histology indicated the most severe degree of stenosis in group C. MSOT images indicated notable differences in tracheal contents of group B and D.</p><p><bold>Conclusion:</bold> This study suggests that PAT/MSOT are potentially valuable non-invasive modality which are capable of evaluating tracheal structure and function <italic>in vivo</italic>.</p>