AUTHOR=Procópio Dielle Pierotti , Kendrick Emanuele , Goldbeck Rosana , Damasio André Ricardo de Lima , Franco Telma Teixeira , Leak David J. , Jin Yong-Su , Basso Thiago Olitta 

TITLE=Xylo-Oligosaccharide Utilization by Engineered Saccharomyces cerevisiae to Produce Ethanol

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.825981

DOI=10.3389/fbioe.2022.825981

ISSN=2296-4185

ABSTRACT=<p>The engineering of xylo-oligosaccharide-consuming <italic>Saccharomyces cerevisiae</italic> strains is a promising approach for more effective utilization of lignocellulosic biomass and the development of economic industrial fermentation processes. Extending the sugar consumption range without catabolite repression by including the metabolism of oligomers instead of only monomers would significantly improve second-generation ethanol production This review focuses on different aspects of the action mechanisms of xylan-degrading enzymes from bacteria and fungi, and their insertion in <italic>S. cerevisiae</italic> strains to obtain microbial cell factories able of consume these complex sugars and convert them to ethanol. Emphasis is given to different strategies for ethanol production from both extracellular and intracellular xylo-oligosaccharide utilization by <italic>S. cerevisiae</italic> strains. The suitability of <italic>S. cerevisiae</italic> for ethanol production combined with its genetic tractability indicates that it can play an important role in xylan bioconversion through the heterologous expression of xylanases from other microorganisms.</p>