AUTHOR=Park Gyuyeon , Kim Ye Chan , Jang Minjeong , Park Hyuna , Lee Hong-Weon , Jeon Wooyoung , Kim Byung-Gee , Choi Kwon-Young , Ahn Jungoh 

TITLE=Biosynthesis of aliphatic plastic monomers with amino residues in Yarrowia lipolytica

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.825576

DOI=10.3389/fbioe.2022.825576

ISSN=2296-4185

ABSTRACT=<p><bold>Introduciton:</bold> The α,ω-diamines (NH<sub>2</sub>-(CH<sub>2</sub>)<sub>n</sub>-NH<sub>2</sub>) and ω -amino fatty acids (NH<sub>2</sub>-(CH<sub>2</sub>)<sub>n</sub>-COOH) have been widely used as building blocks in polymerindustries. Medium- to long-chain (C<sub>8</sub> to C<sub>18</sub>) fatty acid monomers with amino residues are almost exclusively produced <italic>via</italic> chemical processes that generate hazardous waste and induce severe environmental problems, such as global warming and pollution. Here, we present the construction platformstrains of <italic>Yarrowia lipolytica</italic> a cheese-ripening yeast, for direct biotransformation of hydrocarbons into medium- to long-chain α,ω-diamines and ωamino fatty acids using metabolic engineering of endogenous fatty acid ω- and β-oxidation pathways and introducing heterologous ω-transaminase in <italic>Y. lipolytica</italic>.</p><p><bold>Methods:</bold> We deleted six genes encoding the acyl-CoA oxidase (ACO1–6) and four fatty aldehyde dehydrogenase genes (FALDH1-4), which catalyze fatty acid β-oxidation and downstream oxidation of fatty aldehydes in <italic>Y. lipolytica</italic>, respectively. The ω-transaminase from <italic>Chromobacterium violaceum</italic> DSM30191 was introduced into the genome of the Δ<italic>POX</italic> Δ<italic>FALDH</italic> strain under the control of <italic>Y. lipolytica</italic>-derived EXP1 promoters.</p><p><bold>Results and Discussion:</bold> The Δ<italic>POX</italic> Δ<italic>FALDH</italic> strains with ω-CvTA successfully accumulated the corresponding C12 αω-diamines into a shaking culture medium with dodecane or dodecanol. In addition, these strains accumulated C<sub>12</sub> ω-amino fatty acids from dodecanoic acid. With the commercially available α,ω-diacid bioprocess, this yeast biosynthesis producing medium- and longchain α,ω-diamines and ω-amino fatty acids could complete the yeast platform technology generating all medium- and long-chain aliphatic polyamide monomers, α,ω-biofunctionalized with one or both carboxylic acid and amino residues.</p>