AUTHOR=Xu Jinliang , Cui Qingjie , Bu Cuina , Ismail Sherif , Ni Shou-Qing 

TITLE=Partition of Anammox and Nitrifiers Through Bio-Carriers for Full-Scale Sidestream Partial Nitrification–Anammox Plant

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2022

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.819937

DOI=10.3389/fbioe.2022.819937

ISSN=2296-4185

ABSTRACT=<p>This study assessed the activity and community structure in different types of sludge to reveal the partition mechanism of anammox and nitrifiers in a full-scale partial nitrification–anammox plant. Batch experiments confirmed that suspended sludge had higher partial nitrification capacity, and biofilm sludge had higher anammox activity, 16.9 times higher than suspended sludge. qPCR analysis confirmed that the <italic>amoA</italic> gene was mainly present in suspended sludge, and the highest abundance of the <italic>Amx</italic> gene was observed in biofilm sludge, reaching 1.01 × 10<sup>7</sup> copies/ng DNA. High-throughput results revealed that <italic>Nitrosomonas</italic> was the main ammonia-oxidizing bacteria with high activity in suspended sludge, and <italic>Candidatus Brocadia</italic> had the highest abundance of 13.4% in biofilm sludge. This is the exploration of the microbial community of three different sludge types in the full-scale sidestream PN/A system for the first time, which can guide the construction and replication of full-scale PN/A plants.</p>