AUTHOR=Wang Hao , Wang Lin , Zhong Baihua , Dai Zhuojun TITLE=Protein Splicing of Inteins: A Powerful Tool in Synthetic Biology JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.810180 DOI=10.3389/fbioe.2022.810180 ISSN=2296-4185 ABSTRACT=

Inteins are protein segments that are capable of enabling the ligation of flanking extein into a new protein, a process known as protein splicing. Since its discovery, inteins have become powerful biotechnological tools for applications such as protein engineering. In the last 10 years, the development in synthetic biology has further endowed inteins with enhanced functions and diverse utilizations. Here we review these efforts and discuss the future directions.