AUTHOR=Chauhan Vivek , Kaushal Diksha , Dhiman Vivek Kumar , Kanwar Shamsher Singh , Singh Devendra , Dhiman Vinay Kumar , Pandey Himanshu TITLE=An Insight in Developing Carrier-Free Immobilized Enzymes JOURNAL=Frontiers in Bioengineering and Biotechnology VOLUME=10 YEAR=2022 URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.794411 DOI=10.3389/fbioe.2022.794411 ISSN=2296-4185 ABSTRACT=

Enzymes play vital roles in all organisms. The enzymatic process is progressively at its peak, mainly for producing biochemical products with a higher value. The immobilization of enzymes can sometimes tremendously improve the outcome of biocatalytic processes, making the product(s) relatively pure and economical. Carrier-free immobilized enzymes can increase the yield of the product and the stability of the enzyme in biocatalysis. Immobilized enzymes are easier to purify. Due to these varied advantages, researchers are tempted to explore carrier-free methods used for the immobilization of enzymes. In this review article, we have discussed various aspects of enzyme immobilization, approaches followed to design a process used for immobilization of an enzyme and the advantages and disadvantages of various common processes used for enzyme immobilization.