AUTHOR=Cha Jaehyun , Bak Hyeonseon , Kwon Inchan 

TITLE=Hydrogen-fueled CO2 reduction using oxygen-tolerant oxidoreductases

JOURNAL=Frontiers in Bioengineering and Biotechnology

VOLUME=10

YEAR=2023

URL=https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2022.1078164

DOI=10.3389/fbioe.2022.1078164

ISSN=2296-4185

ABSTRACT=<p>Hydrogen gas obtained from cheap or sustainable sources has been investigated as an alternative to fossil fuels. By using hydrogenase (H<sub>2</sub>ase) and formate dehydrogenase (FDH), H<sub>2</sub> and CO<sub>2</sub> gases can be converted to formate, which can be conveniently stored and transported. However, developing an enzymatic process that converts H<sub>2</sub> and CO<sub>2</sub> obtained from cheap sources into formate is challenging because even a very small amount of O<sub>2</sub> included in the cheap sources damages most H<sub>2</sub>ases and FDHs. In order to overcome this limitation, we investigated a pair of oxygen-tolerant H<sub>2</sub>ase and FDH. We achieved the cascade reaction between H<sub>2</sub>ase from <italic>Ralstonia eutropha</italic> H16 (ReSH) and FDH from <italic>Rhodobacter capsulatus</italic> (RcFDH) to convert H<sub>2</sub> and CO<sub>2</sub> to formate using <italic>in situ</italic> regeneration of NAD<sup>+</sup>/NADH in the presence of O<sub>2</sub>.</p>